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(A) In presence of pyruvate during CF serum-starvation, PC detyrosination increased in a dose dependent manner. (B-D) Graphs showing the impact of acetylation inhibition using GM-90257 (B) and MAPK inhibition using inhibitors <t>PD98059</t> (C) and SCH772984 (D) on the detyrosination at PC level. (E-F) Graphs showing the impact of the detyrosination inhibitor LV80 on PC length (E) , number of PC-positive cells (F) and detyrosination level of PC (G) in 24h serum starved colonic fibroblasts cultures. (A, C, D, E, G) Statistical analysis: *p<0.05, **p< 0.01, ****p<0.0001 by two-tailed unpaired t-test.
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(A) In presence of pyruvate during CF serum-starvation, PC detyrosination increased in a dose dependent manner. (B-D) Graphs showing the impact of acetylation inhibition using GM-90257 (B) and MAPK inhibition using inhibitors <t>PD98059</t> (C) and SCH772984 (D) on the detyrosination at PC level. (E-F) Graphs showing the impact of the detyrosination inhibitor LV80 on PC length (E) , number of PC-positive cells (F) and detyrosination level of PC (G) in 24h serum starved colonic fibroblasts cultures. (A, C, D, E, G) Statistical analysis: *p<0.05, **p< 0.01, ****p<0.0001 by two-tailed unpaired t-test.
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(A) In presence of pyruvate during CF serum-starvation, PC detyrosination increased in a dose dependent manner. (B-D) Graphs showing the impact of acetylation inhibition using GM-90257 (B) and MAPK inhibition using inhibitors <t>PD98059</t> (C) and SCH772984 (D) on the detyrosination at PC level. (E-F) Graphs showing the impact of the detyrosination inhibitor LV80 on PC length (E) , number of PC-positive cells (F) and detyrosination level of PC (G) in 24h serum starved colonic fibroblasts cultures. (A, C, D, E, G) Statistical analysis: *p<0.05, **p< 0.01, ****p<0.0001 by two-tailed unpaired t-test.
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(A) In presence of pyruvate during CF serum-starvation, PC detyrosination increased in a dose dependent manner. (B-D) Graphs showing the impact of acetylation inhibition using GM-90257 (B) and MAPK inhibition using inhibitors <t>PD98059</t> (C) and SCH772984 (D) on the detyrosination at PC level. (E-F) Graphs showing the impact of the detyrosination inhibitor LV80 on PC length (E) , number of PC-positive cells (F) and detyrosination level of PC (G) in 24h serum starved colonic fibroblasts cultures. (A, C, D, E, G) Statistical analysis: *p<0.05, **p< 0.01, ****p<0.0001 by two-tailed unpaired t-test.
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(A) In presence of pyruvate during CF serum-starvation, PC detyrosination increased in a dose dependent manner. (B-D) Graphs showing the impact of acetylation inhibition using GM-90257 (B) and MAPK inhibition using inhibitors <t>PD98059</t> (C) and SCH772984 (D) on the detyrosination at PC level. (E-F) Graphs showing the impact of the detyrosination inhibitor LV80 on PC length (E) , number of PC-positive cells (F) and detyrosination level of PC (G) in 24h serum starved colonic fibroblasts cultures. (A, C, D, E, G) Statistical analysis: *p<0.05, **p< 0.01, ****p<0.0001 by two-tailed unpaired t-test.
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(A) In presence of pyruvate during CF serum-starvation, PC detyrosination increased in a dose dependent manner. (B-D) Graphs showing the impact of acetylation inhibition using GM-90257 (B) and MAPK inhibition using inhibitors <t>PD98059</t> (C) and SCH772984 (D) on the detyrosination at PC level. (E-F) Graphs showing the impact of the detyrosination inhibitor LV80 on PC length (E) , number of PC-positive cells (F) and detyrosination level of PC (G) in 24h serum starved colonic fibroblasts cultures. (A, C, D, E, G) Statistical analysis: *p<0.05, **p< 0.01, ****p<0.0001 by two-tailed unpaired t-test.
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(A) In presence of pyruvate during CF serum-starvation, PC detyrosination increased in a dose dependent manner. (B-D) Graphs showing the impact of acetylation inhibition using GM-90257 (B) and MAPK inhibition using inhibitors PD98059 (C) and SCH772984 (D) on the detyrosination at PC level. (E-F) Graphs showing the impact of the detyrosination inhibitor LV80 on PC length (E) , number of PC-positive cells (F) and detyrosination level of PC (G) in 24h serum starved colonic fibroblasts cultures. (A, C, D, E, G) Statistical analysis: *p<0.05, **p< 0.01, ****p<0.0001 by two-tailed unpaired t-test.

Journal: bioRxiv

Article Title: Pyruvate promotes ciliogenesis bypassing IFT88 dependency and attenuates DSS-induced colitis

doi: 10.64898/2025.12.17.694572

Figure Lengend Snippet: (A) In presence of pyruvate during CF serum-starvation, PC detyrosination increased in a dose dependent manner. (B-D) Graphs showing the impact of acetylation inhibition using GM-90257 (B) and MAPK inhibition using inhibitors PD98059 (C) and SCH772984 (D) on the detyrosination at PC level. (E-F) Graphs showing the impact of the detyrosination inhibitor LV80 on PC length (E) , number of PC-positive cells (F) and detyrosination level of PC (G) in 24h serum starved colonic fibroblasts cultures. (A, C, D, E, G) Statistical analysis: *p<0.05, **p< 0.01, ****p<0.0001 by two-tailed unpaired t-test.

Article Snippet: The MEK1/2 inhibitor PD98059 (used at 20μM), ERK1/2 inhibitor SCH772984 (used at 300nM), the inhibitor of pyruvate transport at the plasma membrane AZD3965 (used at 200nM), the microtubule acetylation inhibitor GM-90257 (used at 500nM) and the histone acetyl transferase inhibitors for E1A-associated Protein p300 (Ep300) C646 (used at 25μM) were purchased from MCE (MedChem Express, Monmouth Junction, NJ, USA).

Techniques: Inhibition, Two Tailed Test